Figure 1
From: Lack of correlation between MYCN expression and the Warburg effect in neuroblastoma cell lines
Constitutive expression of MYCN in BE(2)-C, NBL-S and SH-EP neuroblastoma cell lines. (A) Levels of MYCN mRNA in neuroblastoma cell lines. mRNA from neuroblastoma cell lines was examined using real-time PCR for MYCN levels. β 2-microglobulin was used as a control gene. Relative (rel) value represents the abundance of MYCN transcript relative to control gene. (B) BE(2)-C, NBL-S and SH-EP cell lines express different levels of MYCN. Lysates from neuroblastoma cell lines were analysed by Western immunoblotting using an anti-MYCN antibody. α-tubulin was used as a protein loading control. Ratio represents intensity of MYCN relative to α-tubulin.