Figure 5
From: Promoter methylation inhibits BRD7 expression in human nasopharyngeal carcinoma cells
Cytosine methylation inhibits transcription factor binding to its corresponding binding sites in BRD7 promoter. Left panel: Comparison of the nuclear binding capabilities of unmethylated and methylated -353/-337 probes by EMSA. Results are representative of three independent experiments. Excess amounts of unlabeled unmethylated -353/-337, methylated -353/-337 were added as competitors. Right panel: Comparison of the nuclear binding capabilities of unmethylated and methylated -330/-317 probes by EMSA. Excess amounts of cold unmethylated -330/-317, methylated -330/-317 were added as competitors. NP: nuclear protein; Wt: wild type probe; Met: methylated probe; CH3-P: SssI methylase treated probe.