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Figure 3 | BMC Cancer

Figure 3

From: Promoter methylation inhibits BRD7 expression in human nasopharyngeal carcinoma cells

Figure 3

DNA methylation inhibitors 5-Aza-CdR augments endogenous mRNA expression of BRD7 gene and reverses the methylation status of BRD7 promoter. (A) RT-PCR amplification of BRD7 expression in 5–8F cells treated with 0–15 μM 5-Aza-CdR, 150–450 nM TSA, and 7.5 μM 5-Aza-CdR combined with 300 nM TSA. (B) PCR amplification of BRD7 promoter region with methylation- and unmethylation-specific primer by using modified gDNA from 5–8F cells in the presence of vehicle or indicated concentration of 5-Aza-CdR, TSA or 5-Aza-CdR combined with TSA. The presence of a PCR band in the lane "M" indicates methylated promoter fragment, while the presence of a PCR band in the lane "U" indicates unmethylated promoter fragment.

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