Figure 4

GIPC1 RNA expression analysis in normal and neoplastic cell lines. Panel A: Northern blot analysis of total RNA was performed with RNA samples from a human microvascular endothelial cell line (HMEC), normal breast epithelium cell line HBL100 and breast cancer cell lines MCF-7, T47D, SK-BR-3, MDA231, MDA157 and MDA453. A probe for the GAPDH gene was used to normalize expression. Panel B: Densitometry analysis of the Northern blot was performed to quantitate the mRNA expression. The data indicates that the GIPC1 gene is upregulated in breast cancer cell lines.