Figure 3From: Native human autoantibodies targeting GIPC1 identify differential expression in malignant tumors of the breast and ovary Internalization of 27.B1 and 27.F7 antibodies into breast cancer cells. 27.B1(B) and 27.F7 (C) antibodies were internalized into MCF7 cells. Antibody-treated cells were evaluated for intracellular staining with the aid of laser scanning confocal microscopy. Warming of the antibody-bound cells to 37°C for 30 minutes revealed intracellular staining of the MCF7 breast cancer cell line. In contrast, no intracellular staining was visualized with the non-internalizing control antibody (human myeloma IgM).Back to article page