Figure 3

Induction of the apoptosis in vivo. Sections from the tumor-bearing mice treated with liposomal honokiol and DDP (A), liposomal honokiol (B), cisplatin (C), liposome (D) or PBS (E) alone were stained with FITC-dUTP as described in Materials and Methods. Apoptotic nuclei (green) were identified by TUNEL staining with FITC-dUTP and observed under a fluorescence microscope (× 200). The treatment with liposomal honokiol and DDP showed apparent apoptotic cells within the tumors, compared treatment with the control groups. Data for the quantitative assessment of apoptosis was expressed as the mean apoptotic index ± SDs (F). H and L mean liposomal honokiol and liposome alone respectively.