Identification of a novel CHEK2variant and assessment of its contribution to the risk of breast cancer in French Canadian women

Novak, David J; Chen, Long Qi; Ghadirian, Parviz; Hamel, Nancy; Zhang, Phil; Rossiny, Vanessa; Cardinal, Guy; Robidoux, André; Tonin, Patricia N; Rousseau, Francois; Narod, Steven A; Foulkes, William D

doi:10.1186/1471-2407-8-239

Table 1 CHEK2 Primers and Details

From: Identification of a novel CHEK2variant and assessment of its contribution to the risk of breast cancer in French Canadian women

Fragment	Size (bp)	Exon	Amino Acid	Primers (5'->3')	Annealing Temp.(°C)
CHEK2EX01	565	1	1–106	Forward: gaactataggtctgggctgttagg Reverse: tccacctggtaatacaactttctg	57
CHEK2EX02	582	2&3	107–197	Forward: tgccttcttaggctattttcctac Reverse: aaccatattctgtaaggacaggac	56
CHEK2EX04	354	4	198–228	Forward: ctcaagggctttacaatatg Reverse: gaaatgagaaaccaccaatc	54
CHEK2EX05	499	5	229–264	Forward: gaatttcacaatccagggctac Reverse: ctcacaaattcatccatctaagcag	56
CHEK2EX06	632	6	265–282	Forward: tagagctgggtttggaactcag Reverse: agctaggcatgtgtgtgaatg	68
CHEK2EX07	434	7	283–304	Forward: aagaagactgggaagagacctagc Reverse: gcaagcctacattagattctttgg	56
CHEK2EX08	365	8	305–336	Forward: catctcattccttagtttccaactg Reverse: tctgcctaattcagggagtaattc	56
CHEK2EX09	331	9	337–365	Forward: ctgtgagatgtgtgtgttggtaac Reverse: tctggataagagcagtatcacctg	58
CHEK2EX10	546	10	366–420	Forward: ttaatttaagcaaaattaaatgtcc Reverse: ggcatggtggtgtgcatc	54
CHEK2EX11	353	11	421–458	Forward: gctgggattacaagcctaagg Reverse: gaagaaactcccaccacagc	69
CHEK2EX12	541	12	459–487	Forward: ggcctgttaattctggcatactc Reverse: aaaggttgtagcctggccag	67
CHEK2EX13	488	13	488–514	Forward: cctctgggaaggtagaggc Reverse: caatccctagctgtgcttatcg	66
CHEK2EX14	585	14	515–543	Forward: cccccactttactggaagc Reverse: gcaaaaccctgtctctacaaaat	64
CHEK2 R406H Allele Specific	N/A	10	N/A	Forward: ggactgctgggtataacca	54
CHEK2 Long Range	~9,200	10–14	366–543	Forward: cgacggccagtctcaagaagaggactgtctt Reverse: gctatgaccatgcacaaagcccaggttccatc	58
CHEK2 Restriction	546	10	366–420	Forward : ttaatttaagcaaaattaaatgtc Reverse : ggcatggtggtgtgcatc	57
CHEK2 Restriction Nested	202	10	380–420	Forward: catgagaaccttatgtggaaccc Reverse: cctggacaacagagcaagacacat	58
CHEK2 1100delC Sizing	196	10	366–396	Forward:aatagaaactgatctagcctacgtgt Reverse: gaacttcaggcgccaagt	60

Summary of primers, annealing termperatures and PCR amplicon sizes for the 14 coding exons of CHEK2. Additional details are listed for primers used for Long Range PCR, R406H and 1100delC genotyping.

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ISSN: 1471-2407

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