Figure 2

A) Cytological analysis of bladder washing sediment negative for the presence of tumor cells (case 11). B) Positive cytology illustrating a tumor recurrent case during the post-surgical monitoring. × 400, Giemsa staining. C) Comparative MSP results from case 11 in tumor tissue (TCC) and in the exfoliated cells from the correspondent bladder washing for RARB and RASSF1A genes. M – methylated allele; U – unmethylated allele. D) Distribution of MSP results among the third set of samples including 39 archived tumor fragments and 23 matched washouts from 20 urinary bladder cancer patients in post-surgical monitoring and comparison with the cytological analysis.