Figure 5

Expression of BiP, XBP1, and grp94 in CS-treated human lung cells. NHBE cells were exposed to air (Mock), 2R4F cigarette smoke with 35 cc puffs diluted in 500 cc air (2R4F), or Brand B cigarette smoke with 35 cc puffs diluted in 500 cc air for 15 minutes. Cells were then placed in fresh medium and returned to the incubator for the time periods specified prior to RNA extraction. Four separate microarray representing four separate samples were used to analyze each condition. Mean intensity values are shown. * = p < 0.01 when compared to Mock.