Figure 11

Contribution of vapor and particulate phases of CS to XBP1 splicing inhibition in A549 cells. A549 cells were exposed to air (mock treatment), whole cigarette smoke (CS), vapor phase, or particulate 'tar' phase from 2R4F cigarettes as described in the legend for Figure 6, then placed in fresh media with or without 1 uM thapsigargin (THAP) and incubated for the time periods specified. PCR was used to determine the relative amounts of spliced and unspliced XBP1. For each lane the extent of splicing was quantified as described in the Methods section and is presented in additional file 3 – Supplemental Table S3: Suppression of XBP1 splicing by cigarette smoke.