Inhibition of HOC-7 transwell penetration by MMP inhibition is unrelated to the Matrigel coating. A. Effect of GM6001 on different modes of HOC-7 migration. B. Cell morphology and migration mode during scratch-wound healing assay at 10 hrs after wounding. Arrows indicate the borders of the scratch at time 0. HOC-7 cells have epithelial morphology and migrate as a sheet, in contrast to fibroblastic-like HEY and ES-2 cells which undergo single-cell migration. Bars represent the mean ± S.E. *Significantly different from control (p < 0.05; 2-tailed t-test).