Transwell collagen I invasion is MMP dependent. A, Confocal microscopy of biotinylated collagen I-coated transwells shows HEY, ES-2 and OVCA429 cells clearance of collagen I from transwell pores is abrogated by the MMP inhibitor GM6001. Transwell membranes coated with collagen I (0.6 μg/mm2) were biotinylated prior to seeding of cells. Membranes were and fixed and stained with FITC-streptavidin at assay termination. Representative areas recorded at 16× magnification are shownwith a magnified view of the transwell pores shown above. B, Collagen I transwell invasion was abbrogated by MMP inhibition. Invasion assays were terminated at 24 hrs for HEY and 55 hrs for OVCA429 and ES-2. Bars represent the mean ± S.E. *Significantly different from control (p < 0.05; 2-tailed t-test).