Figure 6

Effects of COX-2 knockdown on production of VEGF and IL-8 in NSCLC cell lines A549, H460 and MOR/P. Lung cancer cells were transfected with COX-2 siRNA or control siRNA for 48 h. (A) Expression of COX-2 and actin was detected following transfection for 48 h by Western blotting. (B) IL-8 production was measured by ELISA following transfection of either COX-2 siRNA (solid bar) or control siRNA (open bar). (C) VEGF production was detected by ELISA following transfection of either COX-2 siRNA (solid bar) or control siRNA (open bar). Each bar is the mean ± SEM of three determinations. * P < 0.05.