Bcl-2 expression in human breast. (A) Patients' information and Bcl-2 immunohistochemistry staining on both normal and cancerous breast tissues from human patients. (B) H&E staining and Bcl-2 immunohistochemistry staining showing dark to brown on one of the normal and one of the cancerous human breast tissues. Blue staining represents nuclei. (C) Western blot analysis of Bcl-2 protein expression in primary cultured human breast cells. CAEC stands for cancer epithelial cells; NEC stands for normal epithelial cells; CASC stands for cancer stromal cells; NSC stands normal stromal cells. Equal amounts of isolated protein from whole cell lysate were subjected to immunoblot with anti-Bcl-2 antibodies. β-actin was used as loading control.