Figure 3

Correlation between DLC2 expression and prognosis of HCC. Expression of DLC2 protein was detected in 128 cases of HCC deparaffinized specimens by immunohistochemistry method. HCC specimen of DLC2 high expression confirmed by Western blotting was used as positive controls (a), whereas negative controls were done by omitting the primary antibody (b). The representative positive expression of DLC2 (c) and negative expression of DLC2 (d) in HCC specimens were presented. Kaplan-Meier survival curves for DLC2-positive expression group (n = 33) and DLC2-negative expression group (n = 95) based on results of immunohistochemistry (e). HCC patients with DLC2-negative expression revealed significant poor prognosis than those with DLC2-positive expression, Log-rank test, P = 0.003.