Table 3 Summary of top candidate reference genes identified by geNorm and NormFinder. For the geNorm data, the optimal number and identity of reference genes proven necessary by a Pairwise variation below 0.15, as recommended by Vandesompele et al. [12], is provided in the order given by the program. The stated genes should be viewed per program, and the combination of genes provided by geNorm cannot be used as single entities as optimal reference genes. The number of patients included was: ER+ (n = 11), ER- (n = 4), normal (n = 3) and ER+ cell lines (n = 3)
From: Identification of genes for normalization of real-time RT-PCR data in breast carcinomas
geNorm | NormFinder | |
|---|---|---|
ER+, ER-, normal and cell lines | RPLP0/TBP, PUM1, GUS-B and β-actin | PUM1 PUM1/RPLP0 |
ER+, ER- and normal tissue | TBP/PUM1 and RPLP0 | PUM1 PUM1/RPLP0 |
ER+ and ER- | TBP/PUM1 and RPLP0 | PUM1 PUM1/RPLP0 |
ER+ | RPLP0/TBP, PUM1 and β-actin | PUM1 |
ER- | PUM1/GUS-B and RPLP0 | β-actin |
Normal* | PUM1/GUS-B and TBP | TBP |
ER+ cell lines | TBP/PUM1 | ABL |