Table 1 Summary of candidate reference genes.
From: Identification of genes for normalization of real-time RT-PCR data in breast carcinomas
Symbol | Gene name (Assay ID) | Function | Location | Assay location (amplicon size) | PG¥ | A/CB # |
|---|---|---|---|---|---|---|
RPLP0 NM_053275.3 | Ribosomal protein, large, P0 (Hs99999902_m1) | Structural protein of ribosomes | 12q24.2 | 325* • (105) | + | (-/+) |
TBP NM_003194.3 | TATA box binding protein (Hs00427620_m1) | Transcription factor; DNA-dependent transcription initiation from Pol II promoter | 6q27 | 730 (91) | - | (-/-) |
PUM1 NM_001020658.1 | Pumilio homolog (Drosophila) (Hs00982765_m1) | RNA binding; translation factor. | 1p35.2 | 1757 (68) | - | (-/-) |
ACTB NM_001101.2 | β-actin (Hs99999903_m1) | Cytoskeletal structural protein involved in various types of cell motility | 7p22-p12 | 36* × • (171) | + | (-/+) |
GUS-B NM_000181.1 | Glucoronidase, beta (Hs99999908_m1) | Hydrolase; carbohydrate metabolism. | 7q21.11 | 1816 (81) | - | (-/NI) |
ABL1 NM_005157.3 | v-abl Abelson murine leukemia viral oncogene homolog 1 (Hs00245443_m1) | Protein kinase; regulation of cell cycle, mismatch repair, DNA damage response | 9q34.1 | 85 (54) | - | (-/NI) |
GAPDH NM_002046.3 | Glyceraldehyde-3-phosphate dehydrogenase (Hs99999905_m1) | Oxidoreduct-ase; glucose metabolism | 12p13.31-p13.1 | 158* × • (122) | - | (+/NI) |
B2M NM_004048.2 | β-2-microglobulin (Hs00187842_m1) | Major histocompati-bility complex antigen class 1 receptor activity | 15q21-q22 | 128 • (64) | - | (-/NI) |