Immunohistochemical analyses of tumour hypoxia, proliferation and vascular area. A representative image for each marker is shown (10× magnification; ME180 tumour growing i/m) A) EF-5,B) Ki67,C) CD31,D) LYVE-1.E) Sections were taken on two levels, 100 μm apart to assess intra-tumoural heterogeneity. The correlations between levels 1 (L1) and 2 (L2) are shown. Each point represents an individual tumour (includes data from PyMT tumours; see Additional File 5), and the legend indicates the tumour model and site of growth. The line of identity is shown. Quantitative analysis of two sections from each tumour; bars represent the median percentage of positive staining [(positive pixels/total number of pixels (positive + negative)) × 100] and error bars show the range. F) Each marker according to whether the tumour was grown in the cervix (n = 9) or i/m (n = 9). G-J) Each marker in both growth sites split according to whether the tumour IFP was above (high; n = 5) or below (low; n = 5) the median IFP; F) EF-5, G) Ki67, H) CD31 and I) LYVE-1.