Figure 5

LPA induces dispersal of DLD-1 cancer colonies. Wounds were inflicted as described in Materials and Methods and images were obtained using a Zeiss Axiovert 200 M microscope (10× Achroplan objective, NA 0.25, Ph 1; scale bar = 50 μm). (A) DLD-1 cells undergo morphological changes and individually disperse upon exposure to LPA, compared to treatment with PBS. (B) Mean percent wound closure ± standard deviation measured in the presence or absence of LPA using the CIA method. (C) Images of PBS or LPA pretreated, "wounded" areas after 8 h of incubation. These results indicate that LPA both influences cell morphology (spurs individual behavior) and stimulates overall wound closure, compared to PBS treated cells (collective behavior).