Figure 8

Effects of progestins in combination with E2 on T47-D breast cancer cell migration. (A-D) Cells were treated with the progestins (P, MPA, DRSP all 100 nM; NES, 1 nM) alone or in combination with E2 (10 nM) for 48 h, in the presence or absence of ORG 31710 (ORG – 1 μM). Other cells were transfected with moesin antisense phosphorotioate oligonucleotides (PON) (AS, antisense – 2 μM) or sense PON (S, sense – 2 μM). T47-D cells were scraped out of the cell culture dish and the extent of migration of the remaining cells was assayed in non-proliferating cells in the presence of Ara-C. Representative images of cell migration are shown. (E) Cell migration distances were measured and values are presented as % of control. * = P < 0.05 vs. the corresponding progestin alone; # = P < 0.05 vs. E2 + the corresponding progestin without ORG; † = P < 0.05 vs. E2.