Figure 1

Expression of ANT1 in various human cancer cells and the effect of ANT1 expression vector in human breast cancer cells. A. To evaluate the levels of ANT1 expression levels in human cancer cell lines from various origins (SNU668, SNU719, SNU638, NCI-H358, A549, NCI-H889, SK-HEP-1, SNU449, SNU423, SK-OV-3, SNU8, SNU840, MCF7, MDA-MB-231, and SK-BR-3), total RNA was extracted and subjected to RT-PCR using specific primers for human ANT1 or β-actin as an internal control. B. Cells were transfected with pcDNA-ANT1; 24 hours later, the cells were stained with Annexin V-FITC and PI, and cell death was analyzed by flow cytometry. C. DNA fragmentation was nduceded by ANT1 over-expression Cells were transfected with pcDNA-ANT1 and 24 hours later, the total genomic DNA was extracted and DNA fragmentation was analyzed by 2% agarose gel electrophoresis.