Skip to main content

Table 3 Primer pair and probe of each biomarker for real-time PCR

From: Diagnostic utility of LunXmRNA in peripheral blood and pleural fluid in patients with primary non-small cell lung cancer

Gene

Sequence of selected primer pair (5'-3')

Probe (5'-FAM---TAMRA-3')

Amplicon length (bp)

Ref

LunX

GCCTCATTGTCTTCTACGGGCTGTT;

CTGAGGGCATTTGTCAAGCTTCCT

CAGACCATGGCCCAGTTTGGAGGCCTG

156

This work

CK19

ACTACAGCCACTACTACACGAC;

CAGAGCCTGTTCCGTCTCAAAC

TCTGGCTGCAGATGACTTCCGAACCA

149

[17]

CEA

AATAACGGGACCTATGCCTGTTT;

CCCCAACCAGCACTCCAAT

CATCTGGAACTTCTCCTGGTCTCTCAGCTG

151

This work

VEGF-C

TTCATTCCATTATTAGACGTTCCCT;

GATTATTCCACATGTAATTGGTGGG

CCAGCAACACTACCA CAGTGTCAGGCA

94

[18]

hnRNP A2/B1

GACTGTGTGGTAATGAGGGATCCT;

GCTCAACTACTCTCCCATCAATTGA

CATGGCTGAGGTTGATGCTGCCAT

133

This work

β-actin

TTGCCGACAGGATGCAGAA;

GCCGATCCACACGGAGTACTT

TCATTGCTCCTCCTGAGC

101

This work

  1. Forward primer was shown as upper sequence and reverse primer was the lower sequence in the respective primer pair. The primers and probes for LunX (NM_130852), CEA (NM_004363), hnRNP A2/B1 (NM_031243) and β-actin (NM_001101.2) were designed using the Primer Express Software (Applied Biosystems). All primers were desalted when purified and the probes were HPLC purified.
Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us