Figure 5

Expression of LunX mRNA in peripheral blood decreases shortly following the treatment of NSCLC. Peripheral blood samples from 12 NSCLC patients were collected 1 day before and 7 days after treatment as shown in Table 2. LunX, CK19, CEA, VEGF-C, hnRNP A2/B1 and β-actin mRNA were detected by real-time RT-PCR, and mRNA copy number was determined by reference to the standard curve, as described in methods. "Tb" represents 1 day before treatment and "Ta" represents 7 days after treatment. The copy number of each mRNA was further normalized as the ratio to the copy number of β-actin. For each gene marker, when the copy number was less than 100, it could not be detectable as a negative case. All the negative results of each indicated gene were shown as number undetected. The Wilcoxon Signed Ranks Test was used to analyze the gene expression levels before and after clinical treatment. P < 0.05 (2-tailed test) was considered significant.