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Table 2 Summary of cell line growth characteristics and tumorigenicity

From: Characterization of three new serous epithelial ovarian cancer cell lines

Cell line growth assays TOV-1946 OV-1946 TOV-2223 TOV-112D
Growth characteristic Doubling time (days) +/- S.D.a 1.3 +/- 0.4 2.5 +/- 0.9 2.6 +/- 0.7 1.0 +/- 0.2
  Saturation density (nb cells +/- S.D.)b 3 053 933 +/- 153 933 3 231 400 +/- 962 723 2 536 867 +/-680 852 6 162133 +/- 515 034
  Number of passages to date >210 >210 >150 >200
Spheroid Formation aggregate semi-compact no compact
Migration time for wound filling (h) 24 48 >48 48
Invasion Mean number of cells +/- S.D.c 417 +/- 226 149 +/- 70 257 +/- 79 118 +/- 34
Soft agarose Colony number +/- S.D.d 11 +/- 3 24 +/- 8 16 +/- 4 27 +/- 10
  Colony size large large small large
Low serum Capacity to grow in low serum (1%) conditions yes yes yes yes
Subcutaneous injection in SCID mice Number of mice with tumors (n = 6) 0 0 0 5
  Mean time of tumor appearance (days) N/A N/A N/A 7
Intraperitoneal injection in SCID mice Number of mice with tumors (n = 6) 3 5 0 6
  Mean time of tumor appearance (days) 125 63 N/A 18
  Number of mice with ascitis 2 1 0 2
  1. N/A not applicable
  2. acalculated according to the slope of the linear portion of the growth curve.
  3. brepresents the mean of maximum number of cells counted among the two independent experiments performed in triplicatat
  4. cnumber of cells that migrate through the matrigel insert
  5. dmean of colony numbers per representative area (minimum 5) from two independent experiment performed in duplicate