A) Representative G-banded metaphases from the TOV-2223 (cell 10) cell line. Arrows indicate the abnormal chromosomes, mar: marker chromosome. B) Representative metaphases from TOV-2223 (cell 45) analyzed by SKY. The origin of several marker chromosomes (mar) is defined by SKY analysis. Other examples of G-banded metaphases and the combined inverted-DAPI and SKY images of different cells are presented (see additional Files 1 and 2).