Figure 1

Levels of EF5 following 4 hours of in vivo acute hypoxia exposure. (A) An H+E stained tumour section from a PyMT mouse tumour with EF5 positive hypoxic areas stained in brown, necrotic tumour core is marked with an asterix. Scale bar: 100 μm. (B) Quantification of total EF5 positive staining area (%) in 31 tumours from 11 PyMT mice exposed to AC or AH conditions for 4 hours showed significantly greater median levels of hypoxia in mice exposed to AH than in mice exposed to AC (P < 0.05; *).