Figure 1From: SOCS3 inhibiting migration of A549 cells correlates with PYK2 signaling in vitro Schematic representations of SOCS3 and three mutants with distinct functional domains deleted. The SOCS3 protein is structurally characterized by three distinct functional domains that include a N-terminal kinase inhibitory region (white), a central SH2 domain (black) and a C-terminal homologous region SOCS-box (gray). (a) The SOCS3-SH2 mutant is a full length SOCS3 without the SH2 region, consisting of the KIR and SOCS-box domains. (b) The KIR region is knocked out in the SOCS3-KIR mutant, with the SH2 and SOCS-box domains remained. (c) The SOCS-box mutant contains the SH2 and KIR domains, with the SOCS-box deleted. A myc tag was added to each of these SOCS3 mutants, and exogenous SOCS3 mutants were represented by the detection of myc tag.Back to article page