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Table 1 Primer sequences and restriction enzymes

From: Modified FOLFOX-6 chemotherapy in advanced gastric cancer: Results of phase II study and comprehensive analysis of polymorphisms as a predictive and prognostic marker

Gene

Polymorphisms

Location

Primer

Restriction enzyme

References

TS (Ch 18p11.32)

2R or 3R VNTR in ER*

ER (5'UTR)

Forward: GTGGCTCCTGCGTTTCCCCC

 

16

   

Backward: GCTCCGAGCCGGCCACAGGCATGGCGCGG

  
 

G/C SNP in 3R

5'UTR

Forward:: GTGGCTCCTGCGTTTCCCCC

Hae III

19

   

Backward: GCTCCGAGCCGGCCACAGGCATGGCGCGG

  
 

6 bp insertion(+)/deletion(-)

3'UTR

Forward: CAAATCTGAGGGAGCTGAGT

Dra I

15

   

Backward: CAGATAAGTGGCAGTACAGA

  

GSTP1 (Ch.11q13)

A/G, Ile105Val

Exon5

Forward: CTCTATGGGAAGGACCAGCA

BsmA I

29

   

Backward: TGAGGGCACAAGAAGCCCCT

  

ERCC1 (Ch.19q13.2)

C/T, Asn118Asn

Exon4

Forward: TCATCCCTATTGATGGCTTCTGCCC

BsrD I

30

   

Backward: GACCATGCCCAGAGGCTTCTCATAG

  
 

C8092A

3'UTR

Forward: CAGAGACAGTGCCCCAAGAG

Mbo II

31

   

Backward: GGGCACCTTCAGCTTTCTTT

  

XPD (Ch.19q13.3)

C/A, Arg156Arg

Exon6

Forward: CACACCTGGCTCATTTTTGTAT

Tfi I

32

   

Backward: TCATCCAGGTTGTAGATGCCA

  
 

G/A, Asp312Asn

Exon10

Forward: CTGTTGGTGGGTGCCCGTATCTGTTGGTCT

Sty I

33

   

Backward: (TAATA)TCGGGGCTCACCCTGCAGCACTTCCT

  
 

A/C, Lys751Gln

Exon23

Forward: GCCCGCTCTGGATTATACG

Pst I

33

   

Backward: CTATCATCTCCTGGCCCCC

  

XRCC (Ch.19q13.2)

G/A, Arg399Gln

Exon10

Forward: TTGTGCTTTCTCTGTGTCCA

Msp I

34

   

Backward: TCCTCCAGCCTTTTCTGATA

  
  1. Ch, chromosome; VNTR, variable number tandem repeats; UTR, untranslated region; ER, enhancer region; TS, thymidylate synthase; GSTP, glutathione S- transferase π; ERCC1, excision repair cross-complementation 1; XPD, xeroderma pigmentosum group D; XRCC, X-ray repair cross-complementing group.
  2. *VNTR polymorphism is a double repeat (2R) or triple repeat (3R) of 28-bp sequence in TS 5'UTR.
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BMC Cancer

ISSN: 1471-2407

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