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Figure 5 | BMC Cancer

Figure 5

From: Cervix carcinoma is associated with an up-regulation and nuclear localization of the dual-specificity protein phosphatase VHR

Figure 5

A/: Electrophoretic pattern of semi-quantitative RT-PCR products of VHR mRNA performed with primers specific for VHR (upper panel) and the housekeeping gene HPRT (lower panel) (A). mRNA were prepared from primary keratinocytes from 3 different hysterectomies and from 5 different cervix cancer cell lines as indicated on the panels. B/: CHX chase analysis by Western blot of primary keratinocytes (KN), HeLa, C33 and E6/E7 cell lines. Cells were treated with CHX (50 μg/ml) and were collected immediately thereafter or after 1, 2, 4, 6 or 8 hours for Western blot analysis using anti-VHR and anti-Actin antibodies.

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