Figure 2

A/: Immunohistochemical demonstration of VHR in cervical tissues. a) In normal exocervical tissue, there is a spreaded weak positive staining. b) In L-SIL, the VHR staining is more pronounced but exclusively cytosolic. c) In H-SIL: strong staining of VHR in the cytosol as well as in few nuclei. d) In SCC : strong nuclear staining in an important number of cancerous cells. e) Cervical glandular cells of the normal endocervix show very weak and spreaded staining of VHR. In endocervical adenocarcinoma (f) and AIS (g) there is strong cytoplasmic staining of VHR. Brown color represents VHR specific staining and blue is the staining for nuclei. B/: Immunofluorescent staining, under laser scanning confocal miscroscopy, of cervical biopsies showing a low VHR immunoreactivity and absence of nuclear staining in exocervical epithelium and high VHR expression, nuclear and peri-nuclear localisation in H-SIL and SCC. Toto-3 (blue) was used to stain the nuclei and merged to VHR staining (green).