Figure 3From: High resolution melting analysis for rapid and sensitive EGFR and KRAS mutation detection in formalin fixed paraffin embedded biopsies Difference plots and sequence traces for EGFR exons 20 and 21. Panel A: The difference plot of EGFR HRM exon 20a shows different melting profiles of three positive samples (TX53 in red, TX383 in brown and TX440 in orange). Panel B: A p.S768I in TX53, a p.H773_R776insYNPY in TX383 and a p.D770_H773insGSVD in TX440 mutation are detected by sequencing from the HRM positive samples. Panel C: The difference plot of EGFR HRM exon 21 shows three HRM positive samples (TX23 in red, TX44 in purple and TX324 in orange). Panel D: Sequencing chromatograms show a p.R836R SNP in TX23, a p.R836C in TX44 and a p.L858R mutation in TX324.Back to article page