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Table 1 Primary antibodies for immunohistochemical analysis

From: Skp2 expression is associated with high risk and elevated Ki67 expression in gastrointestinal stromal tumours

Antibody Clone Manufacturer Retrieval Dilution Method
CD117 Polyclonal DAKO, Carpinteria, CA, USA Steam 120°/3' Cytrate buffer pH 6 1:300 ABC
CD34 QBEND10 DAKO, Carpinteria, CA, USA Microwave 98°/30' Cytrate buffer pH 6 1:900 ABC
CK22 Cytokeratin Biomeda, san Francisco, CA, USA Microwave 98°/30' Cytrate buffer pH 6 1:400 ABC
Desmin D33 DAKO, Carpinteria, CA, USA Cook 15' Cytrate buffer pH 6 1:200 ABC
SMA 1A4 Sigma, Saint Louis, MO, USA Microwave 80°/30' Cytrate buffer pH 6 1:60000 ABC
S-100 Polyclonal DAKO, Carpinteria, CA, USA Microwave 80°/30' Cytrate buffer pH 6 1:10000 ABC
NSE BBS/NC/VI DAKO, Carpinteria, CA, USA Cook 15' Cytrate buffer pH 6 1:8000 ABC
Ki67 MIB-1 DAKO, Carpinteria, CA, USA Microwave 98°/60' Cytrate buffer pH 6 1:16000 ABC
p27 Kip1 57 BD Transduction Laboratories, Lexington KY, USA Microwave 98°/60' Cytrate buffer pH 6 1:500 ABC
Jab-1 Polyclonal Santa Cruz Biotechnology, Santa Cruz, CA, USA Microwave 98°/60' Cytrate buffer pH 6 1:200 ABC
Skp2 2C8D9 Zymed LAB, San Francisco, CA, USA Microwave 98°/60' Cytrate buffer pH 6 1:100 ABC