Skp2 expression is associated with high risk and elevated Ki67 expression in gastrointestinal stromal tumours

BMC Cancer

Table 1 Primary antibodies for immunohistochemical analysis

Antibody	Clone	Manufacturer	Retrieval	Dilution	Method
CD117	Polyclonal	DAKO, Carpinteria, CA, USA	Steam 120°/3' Cytrate buffer pH 6	1:300	ABC
CD34	QBEND10	DAKO, Carpinteria, CA, USA	Microwave 98°/30' Cytrate buffer pH 6	1:900	ABC
CK22	Cytokeratin	Biomeda, san Francisco, CA, USA	Microwave 98°/30' Cytrate buffer pH 6	1:400	ABC
Desmin	D33	DAKO, Carpinteria, CA, USA	Cook 15' Cytrate buffer pH 6	1:200	ABC
SMA	1A4	Sigma, Saint Louis, MO, USA	Microwave 80°/30' Cytrate buffer pH 6	1:60000	ABC
S-100	Polyclonal	DAKO, Carpinteria, CA, USA	Microwave 80°/30' Cytrate buffer pH 6	1:10000	ABC
NSE	BBS/NC/VI	DAKO, Carpinteria, CA, USA	Cook 15' Cytrate buffer pH 6	1:8000	ABC
Ki67	MIB-1	DAKO, Carpinteria, CA, USA	Microwave 98°/60' Cytrate buffer pH 6	1:16000	ABC
p27 ^Kip1	57	BD Transduction Laboratories, Lexington KY, USA	Microwave 98°/60' Cytrate buffer pH 6	1:500	ABC
Jab-1	Polyclonal	Santa Cruz Biotechnology, Santa Cruz, CA, USA	Microwave 98°/60' Cytrate buffer pH 6	1:200	ABC
Skp2	2C8D9	Zymed LAB, San Francisco, CA, USA	Microwave 98°/60' Cytrate buffer pH 6	1:100	ABC

ISSN: 1471-2407