Figure 3From: Knockdown of TFIIS by RNA silencing inhibits cancer cell proliferation and induces apoptosis Effects of TFIIS siRNA on proliferation of lung A549 and pancreatic PL45 cells. A549 and PL45 cell lines were subjected to siRNA directed at TFIIS #1–3, Scrambled siRNA (S-siRNA), or Eg5. Cell proliferation represents the fraction of viable cells compared to cells transfected with vehicle alone (1 = 100% viable cells). Caspase-3/7 activity is also depicted and normalized to cell number represented by MTS values. Total activity was then normalized to activity in cells treated with vehicle alone. TFIIS siRNA #2 nearly perfectly overlays TFIIS siRNA #1 in MCF7 cells and is therefore mostly not visible. Data points represent mean and standard error. The assay was performed 72 hours after initial siRNA transfection.Back to article page