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Figure 2 | BMC Cancer

Figure 2

From: Knockdown of TFIIS by RNA silencing inhibits cancer cell proliferation and induces apoptosis

Figure 2

TFIIS siRNA is specific for cancer cells and causes cell death. (A) MCF10A (Black) and MCF7 (White) cell lines were subjected to siRNA directed at TFIIS employing siRNA#3 and proliferation determined 72 hours after treatment. siRNA concentrations and the fraction of cell proliferation normalized to S-siRNA treated cells are indicated (1 = 100% viable cells when treated with S-siRNA). The ratio of cell proliferation between MCF10A and MCF7 cells is shown above the bars. (B) The bar graph represents the number of trypan blue excluded cells. Plated, represents the number of cells initially seeded. Media represents the number of cells when employing unmodified media. Also listed is the number of viable cells after treatment of MCF7 with lipid transfer vehicle identified as "Vehicle" TFIIS siRNA, S-siRNA or Eg5 siRNA. Data points represent mean and standard error. Proliferation was determined 72 hours after siRNA treatment.

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