Clone-specific expression, transcriptional regulation, and action of interleukin-6 in human colon carcinoma cells

BMC Cancer

Table 1 Characteristics of human colon carcinoma cell lines

	Caco-2/AQ	COGA-1A	COGA-13
Log phase doubling time (h)	24	15	48
Alkaline phosphatase (mU/μg cellular protein)	60.2 ± 9.1	2.9 ± 0.1	0.7 ± 0.2
Protein
CK8	+++	+++	+
Vimentin	+	+	+++
Cyclin D1	+	++	+++
p27	+++	++	+/-
mRNA expression
CYP24
basal	+/-	++	+++
after 1,25-(OH)₂D₃ ^a	+++	+++	+++
CYP27B1	+++	+/-	+/-
VDR	+++	+++	++
ER-α	+/-	+/-	+/-
ER-β	+	+	+

For details see [5,19,24]. Protein and mRNA expression in confluent cells were classified by visual inspection of Western blots or RT-PCR gels as: nil, -; low, +; medium, ++; high, +++.
^aafter treatment with 1,25-(OH)₂D₃ (cf. [19])

ISSN: 1471-2407