Figure 6

Activity of NF-kappaB in control and doxycycline-treated MCF7-HER2 cells. MCF7-HER2 cells were plated in duplicate wells at a concentration of 5 × 10⁴ cells per well in 24 well plates. Doxycycline (Dox) or saline (control) was added 16 hours later and incubation continued for additional 48 hours, after which cells received fresh serum-containing medium with or without Dox. Cells were then co-transfected with NF-kappaB-RE-Luc plasmid and pRL-TK, which contains Renilla luciferase sequence under control of TK promoter. 48 hours later cells were harvested and dual-luciferase assay was performed per manufacturer's instructions. Data are presented as the ratio of Firefly/Renilla luciferase actvity × 100.