Figure 1From: Expression of HER-2 in MCF-7 breast cancer cells modulates anti-apoptotic proteins Survivin and Bcl-2 via the extracellular signal-related kinase (ERK) and phosphoinositide-3 kinase (PI3K) signalling pathways Doxycycline-inducible expression of HER-2 protein. Three pTRE-HER2-transfected clones (H1, H2 and H3) and two pTRE-transfected control MCF-7 clones (C1 and C2) were incubated with or without doxycycline. Whole cell protein extracts were collected and evaluated for HER2 expression by western blotting using HER2 antibodies.Back to article page