Table 1 Effect of different concentrations and exposure times of DAC on loss of clonogenicity of human HL-60 myeloid leukemic cells.
From: Importance of dose-schedule of 5-aza-2'-deoxycytidine for epigenetic therapy of cancer
Loss of clonogenicity (%) | ||||
|---|---|---|---|---|
DAC Concentration ng/ml (μM) | Exposure time | |||
2 h | 6 h | 24 h | 48 h | |
10 (0.044) | 11.8 ± 9.8a | 43.9 ± 10.2a | 44.9 ± 15.9a | 69.0 ± 19.9a |
20 (0.088) | ND | ND | 56.1 ± 19.2 | ND |
100 (0.44) | 43.9 ± 7.8 | 74.0 ± 9.8 | 83.9 ± 4.9 | 99.7 ± 0.8 |
200 (0.88) | 56.4 ± 14.8 | 77.2 ± 10.7 | 99.2 ± 2.1 | 100 |
1,000 (4.4) | 61.3 ± 14.7 | 89.3 ± 3.8 | ND | 100 |