Table 1 Primers used in methylation specific PCR and bisulfite genomic sequnecing

Promoter A (methylated)

1711–1842

Forward: 5'-TTC GTC GTA GTT TGC GAA TG

1711–1730

Reverse: 5'-CAA CAC GTA CCT AAA CGC GA

1842-1821

Promoter A (unmethylated)

1710–1843

Forward: 5'-TTT GTT GTA GTT TGT GAA TGG

1710–1730

Reverse: 5'-ACA ACA CAT ACC TAA ACA CAA

1843-1823

Promoter B

Ref. [23]

Real-time Quantitative MSP

Promoter B (Methylated)

1171–1293

Forward: 5'-TTG TCG TAG CGT TCG GTT AC

1171–1190

Reverse: 5'-AAA CTA CAA CGC CCA CAA AA

1293 – 1274

Probe: 5'-Fam-TCG CGG GAC GGA TTC GTT TA

1218–1237

Bisulfite Genomic Sequencing

Promoter B

1141–1454

Forward: 5'-tgg gtg gga ggg tgt tt

1141–1157

Reverse: 5'-cct cac aaa acc cta caa acc t

1454-1433