Figure 3

Bisulfite Genomic Sequencing. Bisulfite genomic sequencing was used to validate the methylation levels measured by real-time quantitative MSP in M805 cells, MDA-MB-231 cells and MCF-7 cells. Sequences of the PCR products (nucleotides no. 1141–1464, Access number: U92868) are mapped, as all the cytidines except those in CpG dinucleotides are converted to uracil (as thymidine in figure) after sodium bisulfite modification. The RFC promoter B is underlined. The number above each cytidine in CpG binucleotide (shown in bold) indicates the percentage of methylated cytidines on the respective site, respectively. The numbers in parenthesis indicate total clonies sequenced and total number of methylated cytidines in each cell line analyzed.