From: Methylation alterations are not a major cause of PTTG1 missregulation
Primer | Sequence (5'-3') | Amplicon | Amplification conditions |
---|---|---|---|
PTTG MF1 | TTTCGGATTGTTAATTGGATTAAC | 168 bp | 1. [95°C for 7'] 2. [95°C for 0'', 60°C for 20'', 72°C for 25 '']x45 3. Melting curve |
PTTG MR1 | AAAAACAAAAACTAAACAACGAA | 2. [95°C for 0'', 60°C for 20'', 72°C for 25 ''] × 45 | |
PTTG UF1 | TTGGATTGTTAATTGGATTAATGG | 166 bp | 3. Melting curve |
PTTG UR1 | AAAAACAAAAACTAAACAACAAA | ||
PTTG MF2 | GTTTTATTTGGTGATTACGTTTACG | 123 bp | 1. [95°C for 7'] |
PTTG MR2 | ACCGCATTCATCTAAAACCG | 2. [95°C for 0'', 60°C for 20'', 72°C for 25 ''] × 45 | |
PTTG UF2 | TTTTATTTGGTGATTATGTTTATGG | 125 bp | 3. Melting curve |
PTTG UR2 | ACAACCACATTCATCTAAAACCAC | ||
PTTG-1F | AAAGTAGCTACCATTCCTGC | 124 bp | 1. [95°C for 7'] 2. [95°C for 30'', 60°C for 30'', 72°C for 30 '']x45 3. [72°C for 3'] |
PTTG-1R | TGCCCTGTAAAAGCAAAAT | 2. [95°C for 30'', 60°C for 30'', 72°C for 30 ''] × 45 | |
PTTG-1 Anc | FL-AATAGCCCAACATAATAGAATCTATTTT-Ph | 3. [72°C for 3'] | |
PTTG-1 Sen | GCTACCATTCCTGCCTTAATA-Cy5 | ||
PTTG-3F | TGCTGACAGGTGCTGGTACT | 128 bp | 1. [95°C for 7'] 2. [95°C for 30'', 60°C for 30'', 72°C for 30 '']x45 3. [72°C for 3'] |
PTTG-3R | AAGAAGCCATAATCCTTAGTTTTCA | 2. [95°C for 30'', 60°C for 30'', 72°C for 30 ''] × 45 | |
PTTG-3 Anc | FL-TTTGAGTCATGCTACTCGAATTACAT-Ph | 3. [72°C for 3'] | |
PTTG-3 Sen | GGTACTTAAATTTCCGATTTTAAC-Cy5 | ||
p16 Forward outer | GTAGGTGGGGAGGAGTTTAGTT | 283 bp | 1. [94°C for 3'] |
p16 Reverse outer | TCTAATAACCAACCAACCCCTCC | 2. [94°C for 30'', 50°C for 30'', 72°C for 30''] × 20 3.[72°C for 4'] | |
p16 Forward inner | GGGGGAGATTTAATTTGG | 190 bp | 1. [94°C for 3'] 2. [94°C for 30'', 50°C for 30'', 72°C for 30''] × 30 3. [72°C for 4'] |
p16 Reverse inner | CCCTCCTCTTTCTTCCTC | 2. [94°C for 30'', 50°C for 30'', 72°C for 30''] × 30 3. [72°C for 4'] | |
qPCR PTTG Fw | AGGCACCCGTGTGGTTGCT | 126 bp | 1. [50°C for 2'] |
qPCR PTTG Rev | TAAGGCTGGTGGGGCATC | 2. [95°C for 2'] | |
qPCR RPL13A Fw | CCTGGAGGAGAAGAGGAAAGAGA | 125 bp | 3. [95°C for 5'', 60°C for 30''] × 40 |
qPCR RPL13A Rev | TTGAGGACCTCTGTGTATTTGTCAA | 4. Melting curve |