Figure 1
From: Methylation alterations are not a major cause of PTTG1 missregulation
Structural search for CpG islands within PTTG1. Design of the primer sets for MSPs. The upper graph represents the GC content expressed as observed/expected along the entire PTTG1 genomic sequence according to the CpG Island Searcher. The middle graph represents the region identified as a putatively functional CpG island, mapping the proximal promoter. This region was subjected to further analysis to refine the critical region and design the necessary MSP primer sets. In the lower graph the critical region is designated as a shadow below the CG percentage. Vertical bars represent each CG pair. Squares represent the different primer sets, designed to amplify if methylated before treatment (black) or unmethylated (grey)