Table 3 Restriction enzyme conditions used for NAT2 polymorphism genotyping.
Polymorphisms | Restriction Enzymes | Temperature and incubation time | Fragment size (pb) | Agarose (%) |
|---|---|---|---|---|
C481T | KpnI (5 U) | 37°C – 4 h 30 | 114 + 426 | 2 |
G590A | TaqI (5 U)* | 65°C – 1 h 30 | 223 + 170 + 147 | 3 |
A803G | DdeI (5 U)** | 37°C – 4 h 30 | 65 + 346 + 25 + 104 | 4 |
G857A | BamHI (5 U) | 37°C – 4 h 30 | 490 + 50 | 2 |