Two-dimensional electrophoretic comparison of metastatic and non-metastatic human breast tumors using in vitrocultured epithelial cells derived from the cancer tissues

Table 5 Identification of significantly changed proteins between the metastase-positive and metastase-negative groups

Spot	Swiss-Prot protein name	Measured Mr/pI	Theor. Mr/pI	Sequence coverage (%)	Peptides sequenced	Score
7305	P42126	27/6.3	28/6	17	47–61 VLVEPDAGAGVAVMK	103
	2,3-trans-Enoyl-CoA				191–200 DTLENTIGHR	48.6
	Isomerase				271–283 DADVQNFVSFISK	18
					289–296 SLQMYLER	20.4
5104	P07203	22/5.9	21/6.1	10	147–155 LITWSPVC*R	5.4
	Glutathione peroxidase 1				165–175 FLVGPDGVPLR	12.1
1606	P06748	37/4.7	33/4.6	26	33–45 VDNDENEHQLSLR	53.6
	Nucleophosmin				55–73 DELHIVEAEAMNYEGSPIK	37.5
					81–101 MSVQPTVSLGGFEITPPVVLR	65.8
					238–246 GPSSVEDIK	20
					276–289 MTDQEAIQDLWQWR	24.9

For each spot we show the number given by PDQuest software, the accession number to the Swiss-Prot database, name, experimentally found (measured) and theoretical (theor.) relative molecular masses in kDa (Mr) and isoelectric points (pI), achieved sequence coverage (%), peptides sequenced by the LC-MS/MS, their composition, position and peptide score. C*: modified cystein detected as carbamidomethyl

ISSN: 1471-2407