Representative 2-DE map. Representative 2-DE map of proteins of primary culture of epithelial breast cancer cells (sample L116). The gels were silver-stained. Solubilized proteins were focused on IPG strips (pH 4–7) and separated in SDS polyacrylamide gradient gels (8–16%). The characteristic proteins are named according to , A = annexin, CK = cytokeratin, eIF = eukaryotic translation factor, FABP = fatty acid-binding protein, GST = glutathione transferase, HSP = heat-shock protein, PDI = protein disulfide isomerase, TCTP = translationally controled tumor protein, TPI = triosephosphate isomerase. Protein spots significantly changed between the metastase-positive and metastase-negative samples are marked and described in Tables 3, 4, 5. The area of gel analyzed using PDQuest is outlined (------).