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Figure 7 | BMC Cancer

Figure 7

From: Activation of Akt is increased in the dysplasia-carcinoma sequence in Barrett's oesophagus and contributes to increased proliferation and inhibition of apoptosis: a histopathological and functional study

Figure 7

Effect of acid and leptin on phosphorylation of Bad. Serum-starved OE33 cells were stimulated with either pH 4.0 acid or control bolus for 3 minutes followed by fresh serum-free medium with or without leptin (10 nM). Cells were formalin fixed 5 minutes after addition of leptin-containing medium for Bad quantitation. Where indicated LY294004 10 μM was added 60 minutes before any stimulation. Phosphorylated and non-phosporylated total Bad were quantified in formalin-fixed cells using ELISA assays. 7a: effect on total Bad. 7b: effect on phsopho-Bad. Results expressed as mean ± SEM, N = 3. OD450 nm/OD 595 nm values represent specific reactivity of the peptide of interest in the ELISA corrected for the number of viable cells in the relevant well. * P < 0.05 vs untreated control, ** P < 0.05 vs acid or leptin alone, *** P < 0.05 vs stimulus in the absence of LY294004.

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