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Figure 6 | BMC Cancer

Figure 6

From: Activation of Akt is increased in the dysplasia-carcinoma sequence in Barrett's oesophagus and contributes to increased proliferation and inhibition of apoptosis: a histopathological and functional study

Figure 6

Effect of acid and leptin on phosphorylation of Akt. Serum-starved OE33 cells were stimulated with either pH 4.0 acid or control bolus for 3 minutes followed by fresh serum-free medium with or without leptin (10 nM). Cells were formalin-fixed and phospho-Akt and total Akt quantified using cell-based ELISA assays. 6a: time course of Akt phosphorylation. Serum-starved cells were treated with control medium or acid bolus for 3 minutes and then control medium or leptin. Results represent the mean of quadruplicate wells. 6b: effect on total Akt levels. 5 minutes after the addition of leptin or control medium cells were formalin fixed and total Akt levels quantified. 6c: effect on phospho-Akt levels. 5 minutes after the addition of leptin or control medium cells were formalin fixed and phsopho-Akt levels quantified. Results expressed as mean ± SEM, N = 3. OD450 nm/OD 595 nm values represent specific reactivity of the peptide of interest in the ELISA corrected for the number of viable cells in the relevant well. * P < 0.05 vs untreated control, ** P < 0.05 vs acid or leptin alone, *** P < 0.05 vs stimulus in the absence of LY294004.

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