Skip to main content
Figure 5 | BMC Cancer

Figure 5

From: Activation of Akt is increased in the dysplasia-carcinoma sequence in Barrett's oesophagus and contributes to increased proliferation and inhibition of apoptosis: a histopathological and functional study

Figure 5

Akt activation is essential for the anti-apoptotic effects of acid and leptin in oesophageal cancer cells. Serum-starved OE33 cells were treated with a pH 4.0 acid or control pulse for three minutes and/or leptin (10 nM) for 24 hours and then apoptosis quantified by an ELISA for intracellular nucleosomes (5a) or caspase-3 activity in cell lysates (5b). Where indicated cells were pretreated with the PI3-kinase inhibitor LY294002 (10 μM) for 60 minutes before any stimulation. Nucleosomes results are expressed as (A405nm – A490nm) values, which represent relative nuclesome concentrations, compared to untreated control cells. Caspase-3 activity is expresses as percentage of untreated control activity. Results expressed as mean ± SEM, N = 3, * P < 0.05 vs untreated control, ** P < 0.05 vs acid or leptin alone, *** P < 0.05 vs stimulus in the absence of LY294004

Back to article page