Figure 1

TXNIP and TRX expression in response to glucose assessed by gene expression profile (GEP) and semi-quantitative PCR. A) MDA-MB-231 cells were grown either in 5 or 20 mM glucose chronically prior to plating. For glucose shift (5/20), cells were plated in 5 mM glucose and shifted to 20 mM at time 0. Cells were harvested at 12 h based on previous growth curves obtained at specified glucose concentration and RNA was isolated, labeled and hybridized to Affymetrix Human U133A microarray chip. Average derived from duplictaes is shown as relative expression of TXNIP and TRX RNAs, respectively. B) TXNIP and TRX RNA message levels were detected by semi-quantitative PCR in MDA-MB-231 cells grown in the same conditions as in A. Average relative levels as compared to control β-actin RNA of TXNIP and TRX RNA messages from duplicates are represented. Representative gel electrophoresis of PCR products obtained from duplicate experiments is shown in the inset.