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Table 3 Effects of oleuropein aglycone on the sensitivity of SKBR3/Tzb100 cells to trastuzumab a

From: Olive oil's bitter principle reverses acquired autoresistance to trastuzumab (Herceptin™) in HER2-overexpressing breast cancer cells

Oleuropein aglycone (μM) TRASTUZUMAB IC30(μg/ml) Sensitization factor (x-fold)b
0 110 -
3.125 3.7 30
6.25 0.13 846
12.5 0.045 > 1,000
25 0.0075 > 1,000
  1. a Data represent the cell viability effects upon simultaneous exposure of HER2-positive and trastuzumab-conditioned (resistant) SKBR3/Tzb100 breast cancer cells to oleuropein aglycone and trastuzumab. Briefly, SKBR3 cells were incubated in serial dilutions of trastuzumab in the absence or presence of a given concentration of oleuropein aglycone. IC30 values are the concentrations of trastuzumab which decreased cell viability by 30%, measured by using a MTT assay as described in "Methods". Values are mean of three independent experiments carried out in triplicate.
  2. b Sensitization factors were obtained by dividing the IC30 value of trastuzumab as single agent by the IC30 values obtained when oleuropein aglycone was concomitantly supplemented.